129S6

129S6/SvEvTac

TRADITIONAL INBRED MICE

Nomenclature: 129S6/SvEvTac

  • Progenitors of this strain used extensively to establish Embryonic Stem (ES) cell lines (e.g., W4) for the production of targeted mutation (knockout) mice
  • Targeted mutation mice developed from 129 agouti ES cell lines can often be maintained with maximum genomic homozygosity by mating chimeras to the 129S6 strain
  • Taconic’s 129 mice are of the Steel (‘S’) subline.

Origin: Taconic received 129S6 inbred model stock from GenPharm International from two sublines originating from Dr. Martin Evan’s colony in 1992. The mice were derived by embryo transfer in 1993. The resulting offspring were heterozygous at Gpi1 for the ‘a’ and ‘c’ alleles. Homozygous Gpi-1c brother x sister mice were selected in the Foundation Colony at F8 and became the common ancestors for all subsequent Taconic breeders. The Taconic foundation colony was at F42 in 2005.

Genetics:

  • Strain Profile: Acy1s, Akp1b, Amy1a, Apoa1b, Car2a, Es1b, Es3c, Esdb, Es11a, Ggca, Glo1a, Got2b, Gpi1c, Gpt1a, Gr1a, Gus-sb, H6pdb, Hbbd, Idh1a, Ldr1a, Mod1a, Mpi1b, Neu1b, Pep3b, Pgm1b, Pgm2a, Pnpa, Trfb, Sod1a
  • Immunology: H2b
  • Other: Ptprcb

Coat Color Loci: Aw

Color: White-bellied Black Agouti

Species: Mouse

Initial Publication: Simpson, EM, et. al; (1997) “Genetic Variation Among 129 substrains and its Importance for ‘Targeted Mutagenesis’ in Mice.” Nature Genetics; Vol. 16(1), p. 19

Animal Diet: NIH #31M Rodent Diet

InVivos’ Health Reports can be found here.

Average litter size: 8

Phenotyping Procedures:

129SVE Physiological Data Summary (May 2003; N=10/group):

Parameter Units 129SVE Males 129SVE Females
Serum Chemistry Avg ± S.D.
Calcium mg/dL 11.8 ± 0.5 10.7 ± 0.6
Phosphorous mg/dL 11.9 ± 1.9 9.9 ± 1.3
Glucose mg/dL 120 ± 23 98 ± 18
Creatinine mg/dL 0.2 ± 0.1 0.1 ± 0.0
BUN mg/dL 31 ± 10 20 ± 2
Total Bilirubin mg/dL 0.1 ± 0.1 0.1 ± 0.0
ALK U/L 199 ± 34 233 ± 25
ALT U/L U/L 19 ± 5 36 ± 38
Total Protein g/dL 5.8 ± 0.2 5.3 ± 0.2
Blood Counts
Erythron
Red Blood Cells X10^6/uL 10.043 ± 0.335 9.4725 ± 0.6297
Nucleated RBC X10^6/uL 0 ± 0 0 ± 0
Hemoglobin g/dL 16.7 ± 0.5 15.9 ± 0.7
Hematocrit % 52.9 ± 1.5 49.0 ± 2.1
MCV fL 53 ± 1 52 ± 2
MCH pG 16.7 ± 0.2 16.8 ± 0.5
MCHC % 31.6 ± 0.3 32.4 ± 0.7
Platelets
Platelets X10^3/uL 799 ± 97 765 ± 59
Leukogram
White Blood Cells x10^3/uL 5.93 ± 0.60 5.30875 ± 1.22240
Neutrophil x10^3/uL 0.571 ± 0.285 0.639 ± 0.307
Bands x10^3/uL 0 ± 0 0 ± 0
Lymphocyte x10^3/uL 5.174 ± 0.541 4.473 ± 1.131
Monocytes x10^3/uL 0.168 ± 0.088 0.124 ± 0.085
Eosinophil x10^3/uL 0.012 ± 0.025 0.073 ± 0.090
Basophils x10^3/uL 0.0062 ± 0.0196 0 ± 0
Others x10^3/uL 0 ± 0 0 ± 0
Organ Weights
Stomach g 0.236 ± 0.038 0.22 ± 0.03
Ileum g 0.630 ± 0.064 0.59 ± 0.13
Colon g 0.127 ± 0.027 0.13 ± 0.02
Lungs g 0.227 ± 0.024 0.19 ± 0.02
Heart g 0.156 ± 0.026 0.12 ± 0.01
Liver g 0.859 ± 0.064 0.70 ± 0.11
Spleen/Pancreas g 0.199 ± 0.029 0.17 ± 0.02
Kidney (L) g 0.164 ± 0.017 0.11 ± 0.01
Kidney (R) g 0.154 ± 0.012 0.11 ± 0.01
Testes (L) g 0.091 ± 0.025
Testes (R) g 0.101 ± 0.018
Ovary (L) g 0.01 ± 0.01
Ovary (R) g 0.02 ± 0.03
Urinalysis
Glucose mg/dL Neg. Neg.
Blood Neg. Neg.
Ph 6.3 ± 0.3 6.4 ± 0.2
Protein mg/dL Trace Trace
Specific Gravity 1.026 ± 0.004 1.016 ± 0.008
Animal Receipt and MaintenanceA quantity of twenty mice (ten males and ten females) was submitted for testing, strain identified as Line 129SVE (129S6/SvEvTac), age 7 weeks. Mice were received at RBU 3, Taconic Biotechnology, Albany NY and acclimated for three days on irradiated NIH #31M rodent diet and sterilized water ad lib, sterile contact bedding (paper chip) and a 12:12 light:dark cycle. All animals appeared normal during this period and routine health surveillance of this colony detected no microbial pathogens. Mice were assigned study unique identification numbers (males 1-10, females 11-20).

 

UrinalysisAll mice were fasted in metabolic cages and an overnight urine sample was collected. Urine analysis was performed using Multistix 10 SG (Bayer). Strips were read and recorded as per the manufacturer’s instructions, and the results are presented in Table 1.

 

Clinical Chemistry and HematologyA terminal blood sample was taken from Carbon Dioxide – anesthetized mice via cardiac puncture. The collected blood was divided into two samples. One sample was treated with EDTA and stored at 4°C for hematological evaluation. Another sample was allowed to clot at 4°C for 30 minutes, and then centrifuged at 7000rpm for 10 minutes, and the serum was decanted and frozen at -80°C for clinical chemistry analysis. A slide smear was made from a single drop of whole blood. Frozen serum, chilled whole blood, and slides were delivered to LabCorp (RTP, NC) for analysis; the results are presented in Table 2. Unless otherwise indicated, serum chemistry data is generated from a Hitachi 717 automated analyzer and hematological data is generated from a Celldyne 3500. WBC differential counts are performed manually. No blood samples were obtained from mouse #16.

 

Necropsy and Organ WeightsAll mice were euthanized and bodyweights were recorded. Representative tissues were collected, weighed, and immersion fixed in 10% Neutral Buffered Formalin. Tissues were delivered to the Taconic lab for histological preparation and evaluation. Tabulation of organ weights is presented in Table 3. The pathologist’s summary and detailed histological descriptions follow.

 

Model 129SVE (strain: 129S6/SvEvTac) Reproductive Data:

Age at Breeder Setup (Sexual Maturity): 7-8 weeks
Summary: The Average Female Breeder produces a litter of 6.4 pups every 5.4 weeks

Criteria Average Std.Dev Low High N=
Newborn Litter Size 6.3 0.88 1 11 112
Weaned Litter Size 6.4 0.93 2 11 107
Weeks from Setup to 1st Litter 3.7 0.5 3 4 25
# of Weeks Breeders Productive 2) 23 8 5 34 25
Features % N=
% of Breeder Pairs Not Producing A Litter (i.e. possible sterility) 0.0% 25 pairs
% of Entire Litters Cannibalized 5.1% 118 litters
Newborn Mortality (excluding Entire Litter Cannibalism) 3.1% 709 newborns
Females as % of Total Weanlings (Sex Ratio) 54% 687 weanlings
Active Breeder Production Efficiency Index (PEI) 1.19 576 Breeder Weeks
1st Litter – Entire Litter Cannibalism Rate 4.0% 25 litters
1st Litter – Newborn Mortality (excluding Entire Litter Cannibalism) 7.7% 143 newborns

Footnotes:

  1. Criteria for Retiring an Active Breeder: No litter or pregnancy observed within six weeks of breeder setup date. Breeder has two consecutive lost litters. Breeder has two consecutive litters of less than 2-3 pups. Breeder found moribund. Breeders generally scheduled for teardown after 30-34 weeks of active breeding
  2. Maximum allowed breeder age in Taconic’s commercial Production colonies is generally 30-34 weeks